PCR Mycoplasma Detection, 20 assays

The PCR Mycoplasma test kit provides a rapid and sensitive PCR method for monitoring your cell cultures for mycoplasma contamination.  Infections usually result in mycoplasma titers of 105 –108 CFU/mL Cell Cultures...

Reagent

409010

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The PCR Mycoplasma test kit provides a rapid and sensitive PCR method for monitoring your cell cultures for mycoplasma contamination.  Infections usually result in mycoplasma titers of 105 –108 CFU/mL

Cell Cultures are used every day as a part of research methods. You rely on these cultures to be healthy, yet up to 35% may be contaminated due to mycoplasma. Mycoplasma contamination affects many cell functions including metabolism, morphology, protein synthesis and cell proliferation - all of which lead to unreliable results as well as lost time and resources. Since contamination is not typically visible by turbidity, routine screening for mycoplasma contamination is essential.

 

References/Citations:

Wu, Y., Yang, Z., Denslin, V., Ren, X., Lee, C. S., Yap, F. L., & Lee, E. H. (2020). Repair of Osteochondral Defects With Predifferentiated Mesenchymal Stem Cells of Distinct Phenotypic Character Derived From a Nanotopographic Platform. The American Journal of Sports Medicine, 0363546520907137.

Parreno, J., Bianchi, V. J., Sermer, C., Regmi, S. C., Backstein, D., Schmidt, T. A., & Kandel, R. A. (2018). Adherent agarose mold cultures: An in vitro platform for multifactorial assessment of passaged chondrocyte redifferentiation. Journal of Orthopaedic Research®, 36(9), 2392-2405.

Warnecke, D., Schild, N. B., Klose, S., Joos, H., Brenner, R. E., Kessler, O., ... & Dürselen, L. (2017). Friction properties of a new silk fibroin scaffold for meniscal replacement. Tribology international, 109, 586-592.

Pallante-Kichura, A. L., Chen, A. C., Temple-Wong, M. M., Bugbee, W. D., & Sah, R. L. (2013). In vivo efficacy of fresh versus frozen osteochondral allografts in the goat at 6 months is associated with PRG4 secretion. Journal of Orthopaedic Research, 31(6), 880-886.

Product Insert (PDF) - Informational use only. Please refer to insert included with product.

Data/Specifications: 

The PCR Mycoplasma Test Kit is adequate to diagnose cell cultures infected with mycoplasmas. Infections usually result in mycoplasma titers of 105 –108 CFU/mL

The kit allows detection of Acholeplasma and Spiroplasma and the following Mycoplasma species:

  • M. fermentans
  • M. hyorhinis
  • M. arginini
  • M. orale 
  • M. salivarium
  • M. hominis
  • M. pulmonis
  • M. arthritidis
  • M. bovis
  • M. pneumoniae
  • M. pirum
  • M. capricolum 


Sensitivity

Type of Mycoplasma

Minimum Concentration Detected

M. FERMENTANS

240 CFU/mL

M. CAPRICOLOM

110 CFU/mL

PENETRANS

200 CFU/mL

HYORHINIS

210 CRU/mL