T1/ST2 (IL-33 R) Mouse, Monoclonal Antibody, 0.5 mL

Mouse T1/ST2 (IL-33 R) monoclonal antibody (clone DJ8) for the identification and purification of murine T helper 2 (Th2) cells and all forms of murine mast cells.   T1/ST2 (also known...

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Mouse T1/ST2 (IL-33 R) monoclonal antibody (clone DJ8) for the identification and purification of murine T helper 2 (Th2) cells and all forms of murine mast cells.

 

T1/ST2 (also known as IL-1 R4 or IL-33Ra) is a transmembrane glycoprotein expressed on mast cells and Th2 cells. It is a selective marker for murine Th2 lymphocytes and plays a role in regulating inflammatory responses. IL-33 is a recently identified member of the IL-1 family of cytokines and is involved in Th2 mediated immune responses. IL-33 mediates its biological effects via T1/ST2 binding. The roles of IL-33 and T1/ST2 (IL-33Ra) have been investigated in many immune responses such as allergy, asthma, rheumatoid arthritis and osteoarthritis.


    References/Citations:

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    Yang, Y., Liu, H., Zhang, H., Ye, Q., Wang, J., Yang, B., ... & Lu, B. (2017). ST2/IL-33-dependent microglial response limits acute ischemic brain injury. Journal of Neuroscience, 3233-16.

    Madouri, F., Chenuet, P., Beuraud, C., Fauconnier, L., Marchiol, T., Rouxel, N., ... & Marquant, Q. (2017). Protein kinase Cθ controls type 2 innate lymphoid cell and TH2 responses to house dust mite allergen. Journal of Allergy and Clinical Immunology, 139(5), 1650-1666.

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    Yoon, J., Leyva-Castillo, J. M., Wang, G., Galand, C., Oyoshi, M. K., Kumar, L., ... & Kuchroo, V. K. (2016). IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization. Journal of Experimental Medicine, 213(10), 2147-2166.

    Galand, C., Leyva-Castillo, J. M., Yoon, J., Han, A., Lee, M. S., McKenzie, A. N., ... & Geha, R. S. (2016). IL-33 promotes food anaphylaxis in epicutaneously sensitized mice by targeting mast cells. Journal of Allergy and Clinical Immunology, 138(5), 1356-1366.

    Bando JK, Liang HE, Locksley RM (2015) Identification and distribution of developing innate lymphoid cells in the fetal mouse intestine. Nat Immunol. 2015 Feb;16(2):153-60

    Filbey KJ, Grainger JR, Smith KA, Boon L, van Rooijen N, Harcus Y, Jenkins S, Hewitson JP, Maizels RM (2014) Innate and adaptive type 2 immune cell responses in genetically controlled resistance to intestinal helminth infection. Immunol Cell Biol. 2014 May-Jun;92(5):436-48.

    Biethahn, K., Orinska, Z., Vigorito, E., Goyeneche‐Patino, D. A., Mirghomizadeh, F., Föger, N., & Bulfone‐Paus, S. (2014). miRNA‐155 controls mast cell activation by regulating the PI3Kγ pathway and anaphylaxis in a mouse model. Allergy, 69(6), 752-762.

    Connor, L. M., Tang, S. C., Camberis, M., Le Gros, G., & Ronchese, F. (2014). Helminth-conditioned dendritic cells prime CD4+ T cells to IL-4 production in vivo. The Journal of Immunology, 193(6), 2709-2717.

    Beale, J., Jayaraman, A., Jackson, D. J., Macintyre, J. D., Edwards, M. R., Walton, R. P., ... & Bartlett, N. W. (2014). Rhinovirus-induced IL-25 in asthma exacerbation drives type 2 immunity and allergic pulmonary inflammation. Science translational medicine, 6(256), 256ra134-256ra134.

    Matta, B. M., Lott, J. M., Mathews, L. R., Liu, Q., Rosborough, B. R., Blazar, B. R., & Turnquist, H. R. (2014). IL-33 is an unconventional alarmin that stimulates IL-2 secretion by dendritic cells to selectively expand IL-33R/ST2+ regulatory T cells. The Journal of Immunology, 193(8), 4010-4020.

    Furusawa, J. I., Moro, K., Motomura, Y., Okamoto, K., Zhu, J., Takayanagi, H., ... & Koyasu, S. (2013). Critical role of p38 and GATA3 in natural helper cell function. The Journal of Immunology, 191(4), 1818-1826.

    Imai, Y., Yasuda, K., Sakaguchi, Y., Haneda, T., Mizutani, H., Yoshimoto, T., ... & Yamanishi, K. (2013). Skin-specific expression of IL-33 activates group 2 innate lymphoid cells and elicits atopic dermatitis-like inflammation in mice. Proceedings of the National Academy of Sciences, 110(34), 13921-13926.

    Lipsky, B. P., Toy, D. Y., Swart, D. A., Smithgall, M. D., & Smith, D. (2012). Deletion of the ST2 proximal promoter disrupts fibroblast‐specific expression but does not reduce the amount of soluble ST2 in circulation. European journal of immunology, 42(7), 1863-1869.

    Bartemes, K. R., Iijima, K., Kobayashi, T., Kephart, G. M., McKenzie, A. N., & Kita, H. (2012). IL-33–Responsive Lineage− CD25+ CD44hi Lymphoid Cells Mediate Innate Type 2 Immunity and Allergic Inflammation in the Lungs. The Journal of Immunology, 188(3), 1503-1513.

    Seol, D., McCabe, D. J., Choe, H., Zheng, H., Yu, Y., Jang, K., & Martin, J. A. (2012). Chondrogenic progenitor cells respond to cartilage injury. Arthritis & Rheumatism, 64(11), 3626-3637.

    Doherty, T. A., Khorram, N., Chang, J. E., Kim, H. K., Rosenthal, P., Croft, M., & Broide, D. H. (2012). STAT6 regulates natural helper cell proliferation during lung inflammation initiated by Alternaria. American Journal of Physiology-Lung Cellular and Molecular Physiology, 303(7), L577-L588.

    Turnquist, H. R., Zhao, Z., Rosborough, B. R., Liu, Q., Castellaneta, A., Isse, K., ... & Thomson, A. W. (2011). IL-33 expands suppressive CD11b+ Gr-1int and regulatory T cells, including ST2L+ Foxp3+ cells, and mediates regulatory T cell-dependent promotion of cardiac allograft survival. The Journal of Immunology, 187(9), 4598-4610.

    Anthony, R. M., Kobayashi, T., Wermeling, F., & Ravetch, J. V. (2011). Intravenous gammaglobulin suppresses inflammation through a novel TH2 pathway. Nature, 475(7354), 110-113.

    Navarro, S., Cossalter, G., Chiavaroli, C., Kanda, A., Fleury, S., Lazzari, A., ... & Julia, V. (2011). The oral administration of bacterial extracts prevents asthma via the recruitment of regulatory T cells to the airways. Mucosal immunology, 4(1), 53-65.

    Ohno, T., Oboki, K., Morita, H., Kajiwara, N., Arae, K., Tanaka, S., & Nakae, S. (2011). Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. PLoS One, 6(4), e18404.

    Föger, N., Jenckel, A., Orinska, Z., Lee, K. H., Chan, A. C., & Bulfone-Paus, S. (2011). Differential regulation of mast cell degranulation versus cytokine secretion by the actin regulatory proteins Coronin1a and Coronin1b. The Journal of experimental medicine, 208(9), 1777-1787.

    Schulze, J., Bickert, T., Beil, F. T., Zaiss, M. M., Albers, J., Wintges, K., & Schinke, T. (2011). Interleukin‐33 is expressed in differentiated osteoblasts and blocks osteoclast formation from bone marrow precursor cells. Journal of Bone and Mineral Research, 26(4), 704-717.

    Rankin, A. L., Mumm, J. B., Murphy, E., Turner, S., Yu, N., McClanahan, T. K., ... & Pflanz, S. (2010). IL-33 induces IL-13–dependent cutaneous fibrosis. The journal of immunology, 184(3), 1526-1535.

    Cunnusamy, K., Chen, P. W., & Niederkorn, J. Y. (2010). IL-17 promotes immune privilege of corneal allografts. The Journal of Immunology, 185(8), 4651-4658.

    Alves-Filho, J. C., Sônego, F., Souto, F. O., Freitas, A., Verri Jr, W. A., Auxiliadora-Martins, M., & Liew, F. Y. (2010). Interleukin-33 attenuates sepsis by enhancing neutrophil influx to the site of infection. Nature medicine, 16(6), 708-712.

    Moro, K., Yamada, T., Tanabe, M., Takeuchi, T., Ikawa, T., Kawamoto, H., & Koyasu, S. (2010). Innate production of TH2 cytokines by adipose tissue-associated c-Kit+ Sca-1+ lymphoid cells. Nature, 463(7280), 540-544.


    IL-33 induces IL-13-dependent cutaneous fibrosis.
    Rankin A, et al. Journ. of Immunol., 2010; 184(3):1526-35.

    IL-33 Enhances Lipopolysaccharide-Induced Inflammatory Cytokine Production from Mouse Macrophages by Regulating Lipopolysaccharide Receptor Complex
    Quentin Espinassous et al., J. Immunol., Jul 2009; 183: 1446 - 1455.

    Interleukin-1 receptor-related protein ST2 suppresses the initial stage of bleomycin-induced lung injury N. Mato et al., Eur. Respir. J., Jun 2009; 33: 1415 - 1428.

    IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex.
    Chackerian A, et al. Journ. of Immunol., 2007; 179(4):2551-5.

    IL-17 Promotes Immune Privilege of Corneal Allografts Khrishen Cunnusamy, Peter W. Chen, and Jerry Y. Niederkorn J. Immunol., Oct 2010; 185: 4651 - 4658.

    Predominance of Th2 response in human abdominal aortic aneurysm: Mistaken identity for IL-4-producing NK and NKT cells? Chan WL, et al. Cellular Immun (2005) 233:109-114

    Changes in systemic type 1 and type 2 immunity in normal pregnancy and pre-eclampsia may be mediated by natural killer cells Borzychowski, A.M. et al., Eur J Immunol (2005) 35:3054-3063.

    Atherosclerotic Abdominal Aortic Aneurysm and the Interaction Between Autologous Human Plaque-Derived Vascular Smooth Muscle Cells, Type-1 NKT, and Helper T-Cells Chan, W.L. et al., Circ Res (2005) 96:675-683

    NKT cell subsets in infection and inflammation Chan WL, et al. Immun Lett (2003) 85:159-163

    Regulation of ST2L expression on T helper (Th) type 2 cells Carter, R.W. et al., Eur. J. Immunol. (2001) 31:2979-2985

    Human IL-18 Receptor and ST@L Are Stable and Selective markers for the Respective Type 1 and Type 2 Circulating Lymphocytes Chan WL, et al. J Immunol. 2001 Aug 1; 167(3):1238-44.

    Product Insert (PDF) - Informational use only. Please refer to insert included with product.

    Data/Specifications: 

    • Rat anti-Mouse T1/ST2 purified mAb
    • Clone: DJ8
    • IgG Subclass: IgG1 (light chain not isotyped)
    • Form: The antibody is supplied in PBS, with 0.1% sodium azide as preservative and purified over protein G-sepharose. The characteristics of each lot are tested by FACS analysis with bone marrow derived mast cells.
    • Specificity: This clone recognizes the membrane anchored murine T1M protein on the surface of T helper 2 cells and mast cells. T1M appears on fetal blood derived mast cell progenitors before they express the Fce RI, on IL-3-dependent bone marrow derived mast cells and on mature peritoneal mast cells. The antibody detects T1S protein consisting only of the extracellular portion of the protein, which is secreted from growth factor and proinflammatory cytokine stimulated murine fibroblasts.
    • Immunogen: Eukariotically expressed fusion protein of mouse T1 ectodomain and human immunoglobulin Fc domain.